Introduction to Electron Microscopy
Eva Nogales (UC Berkeley): Introduction to Electron Microscopy
iBIOLOGY -
Transmission electron microscopy (TEM) offers the possibility of
visualizing biological structures at resolution well beyond that of
light microscopy. Whether you are interested in the ultrastructure of
cells and organelles, or in the detailed molecular structure of
biological macromolecules, different modalities of TEM can generally be
applied to your system of interest.
The lecture reviews the
physical principles underlying image formation by the interaction of
electrons with matter, introduces you to basic and advanced instruments
and to sample preparation techniques. Using a number of biological
examples from work in the Nogales lab, the lecture then describes the
capabilities of the TEM methodology. Special emphasis is placed on the
image processing methods used to obtain three-dimensional information
from TEM data. See more at http://www.ibioseminars.org