ICB5754 - From Cloning to Protein Analysis - Methods and Strategies

Programação

• Geral

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    Avisos Fórum
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    Programa Geral do Curso Arquivo
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    Tutorial para as práticas Arquivo

• Class 1 - KNOW YOUR TARGET
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    Pymol Tutorial Arquivo
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    Class 1 - Know your target Arquivo
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    pETManual Arquivo

• Class 2 - CONSTRUCT DESIGN
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    Lecture 2 -Construct Design Arquivo
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    Snapgene video URL
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    Vetores Pasta

• Class 3 - PROTEIN EXPRESSION AND PURIFICATION
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    Lecture 3 - ProteinExpression Arquivo
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    Lecture 4 - Protein purification Arquivo
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    Manuais GE -Sample preparation and purification Pasta
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    Calbiochem Buffers Booklet Arquivo
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    E. coli -células de expressão Pasta
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    Biological buffers Arquivo

• Class 4 - PROTEIN PURIFICATION AND ANALYSIS
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    Protein production USP 2022 Lecture 5 and 6 Arquivo
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    GE Spectrophotometry Arquivo
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    Protein concentration measurement Arquivo
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    Thermal shift NatureProtocols Arquivo
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    Wang et al SciRep 2017 RefoldingScreen Arquivo