clear load modelo2 mutante=model; mutante = changeRxnBounds(mutante,'EX_glc(e)',-8,'l'); mutante = changeRxnBounds(mutante,'ATPM',8.39,'b'); %mutante = changeRxnBounds(mutante,'Biomass_Ecoli_core_w_GAM',0.1,'l'); mutante = addReaction(mutante,'sintPHB',{'accoa[c]','nadph[c]','h[c]','3HB','coa[c]','nadp[c]'},[-2 -1 -1 1 2 1],false,0,70); mutante = addExchangeRxn(mutante,{'3HB'},-20,70); mutante = changeObjective(mutante,'sintPHB'); solucao = optimizeCbModel(mutante,'max'); polimero = solucao.f cresc = findRxnIDs(mutante,{'Biomass_Ecoli_core_w_GAM'}); taxa = solucao.x(cresc)